Journal: Clinical and Experimental Immunology

Article Title: The effect of human immunodeficiency virus-1 on monocyte-derived dendritic cell maturation and function

doi: 10.1111/j.1365-2249.2012.04628.x

Figure Lengend Snippet: Human immunodeficiency virus (HIV)-1 infection of monocyte-derived dendritic cells (MDDC) inhibits antigen presentation to autologous CD8+ T cells. HIV-1CS204-infected immature MDDC (iMDDC) were pulsed with a cytomegalovirus, Epstein–Barr and influenza viruses (CEF) peptide pool and then co-cultured with autologous 5 mM CellTrace™ carboxyfluorescein succinimidyl ester (CFSE)-stained peripheral blood mononuclear cells (PBMC) at a ratio of 1:10 for 7 days. While preincubation of uninfected iMDDC induced the proliferation of autologous monocyte depleted PBMC, HIV-1CS204 infection of iMDDCs impaired the presentation of a CEF peptide pool as measured by CFSE dilution. Results are expressed as means ± standard deviation. *P = 0·02 by paired t-test (n = 6).

Article Snippet: MDDCs, differentiated and infected as above, were pulsed for 3 h with 3 μg/ml of a CEF peptide pool containing 23 human leucocyte antigen (HLA)-ABC-restricted T cell epitopes from human cytomegalovirus, Epstein–Barr and influenza viruses (CEF) (Anaspec Inc., Fremont, CA, USA).

Techniques: Virus, Infection, Derivative Assay, Immunopeptidomics, Cell Culture, Staining, Standard Deviation

Journal: Frontiers in Immunology

Article Title: A SARS-CoV-2 NSP7 homolog of a Treg epitope suppresses CD4+ and CD8+ T cell memory responses

doi: 10.3389/fimmu.2023.1290688

Figure Lengend Snippet: Effect of SARS-CoV-2-NSP7 peptide on CD8+ T cell response in PBMCs from healthy donors stimulated with CEF peptides. PBMCs from healthy donors were stimulated with 0.5 µg/ml of CEF alone or in combination with FV621 or increasing concentrations of 289, 289z or SARS-CoV-2 NSP7 peptide (10, 20 and 40μg/ml). Proliferation of CD8+ T cells were measured six days post-stimulation by flow cytometry based on CFSE dilution. (A) Left panel shows the gating of CD8+ T cells. In the right panel, representative flow plots display the dose effect on the proliferation of CD8+ T cells resulting from increasing concentrations of SARS-CoV-2-NSP7-homolog peptide. (B, C) Graphs show the (%) proliferation of CD8+ T cells for each peptide tested in the assay, compared against CEF stimulation alone so that percent inhibition can be calculated or inferred. FV621 was used as a positive control as it has demonstrated consistent suppression of CEF response across donors in our previous publication . Original data is presented in panel B, and data normalized for CEF stimulation as 100% proliferation in panel C. The percent inhibition of CD8+ T cell proliferation for each peptide at each concentration tested in the assay compared to CEF stimulation alone. Data are combined from five donors in the experiment. P values * = <0.05, ** = <0.01 and *** = <0.0002 represent statistical significance vs . CEF stimulation alone using a two-tailed t test.

Article Snippet: To determine the effects of NSP7-289 on CD8+ T cell recall responses, and demonstrate that both CD4+ and CD8+ T cells can be suppressed by the Treg epitopes, PBMCs from healthy donors were stimulated with 0.5 μg/ml of a mixture of MHC class I peptides derived from cytomegalovirus, Epstein-Barr virus, and influenza virus designated CEF (CEF peptide pool, Mabtech, cat no. 3615-1), alone or with the of test peptides.

Techniques: Flow Cytometry, Inhibition, Positive Control, Concentration Assay, Two Tailed Test

Journal: Cancer Immunology, Immunotherapy

Article Title: How frequently are predicted peptides actually recognized by CD8 cells?

doi: 10.1007/s00262-016-1840-7

Figure Lengend Snippet: List of CEF peptides and their restricting MHC Class I alleles as originally defined

Article Snippet: The individual CEF peptides, with amino acid sequences specified in Table , were purchased from Panatecs (Heilbronn, Germany) and were of more than 95 % purity.

Techniques: Virus, Sequencing

Journal: Cancer Immunology, Immunotherapy

Article Title: How frequently are predicted peptides actually recognized by CD8 cells?

doi: 10.1007/s00262-016-1840-7

Figure Lengend Snippet: Predicted versus actually detected responses for all donors . Forty-two donors were tested for the 32 individual CEF peptides. a Of the predicted 241 recall responses, in 122 instances recall responses of various magnitudes were detected, graded as dominant, subdominant, and cryptic, as specified in the Text. In 119 instances no response was detected. b Predicted versus unpredicted responses among positives. Fifty-seven unpredicted responses (shown in green ) were seen versus the 122 predicted responses. Within the unpredicted responses 20 (35 %) were dominant, 20 (35 %) were subdominant, and 17 (30 %) were cryptic

Article Snippet: The individual CEF peptides, with amino acid sequences specified in Table , were purchased from Panatecs (Heilbronn, Germany) and were of more than 95 % purity.

Techniques: